Previous research in our lab involved small RNA-mediated regulation of gene expression both in Drosophila and human. We used the maternal-to-zygotic transition in Drosophila embryos to investigate the intracellular dynamics of small RNAs, tRNA-derived fragments (tRFs), miRNAs and piRNAs. Fractionation of intracellular contents based on A254 reading allowed us to establish a correlation between various types of small RNAs and cellular translational machinery. Funded by three seperate TUBITAK projects, our research yielded interesting results in that tRFs are associated primarily with non-polysomal fractions whereas other small RNAs appear to have different localizations. We also reported the developmentally differential regulation of tRFs, which were detected primarily from the 5'-ends of tRNAs (TUBITAK KBAG 104T144, 209T116, 210T006).
In another TUBITAK Project, we investigated the miRNA prolifes of Jurkat cells treated with camptothecin, a chemotherapeutic drug. Interestingly, we identified a CPT-resistant sub-population in the Jurkat cell line that has a different miRNA prolife despite the exposure to a similar drug concentrations. To this extent, we reported 6 miRNAs that are inversely expressed in the CPT-resistant and susceptible sub-populations (TUBITAK KBAG 107T475).