Previous research in our lab involved small RNA-mediated regulation of gene expression both in Drosophila and human. We used the maternal-to-zygotic transition in Drosophila embryos to investigate the intracellular dynamics of small RNAs, tRNA-derived fragments (tRFs), miRNAs and piRNAs. Fractionation of intracellular contents based on A254 reading allowed us to establish a correlation between various types of small RNAs and cellular translational machinery. Funded by three seperate TUBITAK projects, our research yielded interesting results in that tRFs are associated primarily with non-polysomal fractions whereas other small RNAs appear to have different localizations. We also reported the developmentally differential regulation of tRFs, which were detected primarily from the 5′-ends of tRNAs (TUBITAK KBAG 104T144, 209T116, 210T006).
In another TUBITAK Project, we investigated the miRNA prolifes of Jurkat cells treated with camptothecin, a chemotherapeutic drug. Interestingly, we identified a CPT-resistant sub-population in the Jurkat cell line that has a different miRNA prolife despite the exposure to a similar drug concentrations. To this extent, we reported 6 miRNAs that are inversely expressed in the CPT-resistant and susceptible sub-populations (TUBITAK KBAG 107T475).
Completed Projects:
TUBITAK KBAG 104T144 (Career Project, 2005-2010, PI)
TUBITAK KBAG 107T475 (1001, 2007-2010, PI)
TUBITAK KBAG 209T116 (1001, 2010-2011, PI)
TUBITAK KBAG 210T006 (1001, 2011-2014, PI)
TUBITAK KBAG 113Z371 (1001, 2013-2016, PI)
TUBITAK KBAG 216Z137 (1002, 2017-2018, PI)
TUBITAK KBAG 215Z081 (1001, 2016-2019, PI)
TUBITAK KBAG 117243 (1001, 2017-2020, PI)
TUBITAK KBAG 119Z926 (1002, 2020-2021, PI)
TUBITAK KBAG 220Z018 (1002, 2021-2022, PI)
TUBITAK KBAG 217Z234 (COST CA16120, 2018-2021, PI)
TUBITAK KBAG 220Z088 (1002, 2021-2022, PI is Bilge Yaylak, a PhD student)
TUBITAK KBAG 219Z208 (1001, 2020-2023, PI)
TUBITAK KBAG 122Z759 (1002, 2022-2023, PI)
TUBITAK KBAG 119Z611 (COST CA18127, 2019-2024, PI)
TUBITAK KBAG 220Z074 (COST CA19138, 2021-2025, PI)
TUBITAK UPAG 122N741 (Bosphorus (TUBITAK-France) bilateral project, 2023-2025, PI)
TUBITAK KBAG 121Z299 (1001, 2021-2026, PI)
P.S. Internally university-funded projects (BAP) are not listed.